Journal: iScience

Article Title: Single-cell temporal analysis of natural dengue infection reveals skin-homing lymphocyte expansion one day before defervescence

doi: 10.1016/j.isci.2022.104034

Figure Lengend Snippet: Dynamics of immune cell populations during DENV infection (A) Summary of PBMC samples from two DENV-infected patients with different severities: DF and DHF, across four time points: two days before defervescence (Day −2), one day before defervescence (Day −1), defervescence (Def), and two weeks after defervescence (Wk2). Two healthy PBMCs from independent sources were also included (see ). (B) Uniform Mani-fold Approximation and Projection (UMAP) plot showing integrated single-cell PBMC profiles from the two patients and from two healthy controls, colored by cell types. (C) Relative abundances of key immune cell populations in each sample. DF = dengue fever; DHF = dengue hemorrhagic fever; HC = healthy control; DCs = dendritic cells; pDCs = plasmacytoid dendritic cells; and RBCs = red blood cells (see also Figure S1 ; and ).

Article Snippet: As part of the DENFREE initiative ( https://cordis.europa.eu/project/id/282378/results ) , we obtained the peripheral blood mononuclear cells (PBMCs) from two Thai male adult donors, both diagnosed with secondary DENV-4 infection with DF and DHF severities, aged 35 and 20 years old, and the viral loads of 1.76 x 10 6 and 1.77 x 10 7 , respectively ( ).

Techniques: Infection, Control

Journal: iScience

Article Title: Single-cell temporal analysis of natural dengue infection reveals skin-homing lymphocyte expansion one day before defervescence

doi: 10.1016/j.isci.2022.104034

Figure Lengend Snippet: Time-course transcriptomic profiling reveals detailed molecular events in the febrile phase of DENV infection (A) Principal Component Analysis (PCA) of overall transcriptomic patterns among the ten PBMC samples. Colors represent the time points of DENV infection; shapes represent different severities. (B) Heatmap showing the relative expression of highly variable genes (HVGs), from the union of the top 500 genes in PC1 and PC2 from (A) (see ). (C) Dotplots illustrating the relative expression of HVGs over the course of DENV infection. The biological processes (BPs) of HVGs that are common across the four cell types are in red; monocyte-specific BPs are in blue, and B cell-specific BPs are in green. The relative expression is indicated by the color intensity (see ). (D) UMAP feature plots of the relative signature scores of HVGs that are associated in that particular BPs (see also Figure S4 ).

Article Snippet: As part of the DENFREE initiative ( https://cordis.europa.eu/project/id/282378/results ) , we obtained the peripheral blood mononuclear cells (PBMCs) from two Thai male adult donors, both diagnosed with secondary DENV-4 infection with DF and DHF severities, aged 35 and 20 years old, and the viral loads of 1.76 x 10 6 and 1.77 x 10 7 , respectively ( ).

Techniques: Infection, Expressing

Journal: iScience

Article Title: Single-cell temporal analysis of natural dengue infection reveals skin-homing lymphocyte expansion one day before defervescence

doi: 10.1016/j.isci.2022.104034

Figure Lengend Snippet: Functional characterisation of T cell subpopulations during DENV infection (A) UMAP plots of integrated T cell transcriptome profiles from the ten PBMC samples (see also Figure S6 and ). (B) Relative abundances of Effector T cells (upper panel) and Naive/Memory T cells (lower panel). (C) Relative expression of the top 20 differentially expressed genes (DEGs) between each of the subpopulations and the rest of effector-like T cells. (D) Average expression of tissue-homing genes in Effector T cells. The dot sizes represent the proportion of cells expressing the genes. (E) UMAP plots showing the Effector CD8 (upper panel) and CD4 (lower panel) T cells. Black lines represent pseudotime constructed by Monocle3 ( Cao et al., 2019 ), by setting Effector CD8-3 and Naive CD4 as roots (see also Figure S13 ). (F) Pseudotime kinetics of SELPLG , and ITGAE from the roots in Effector CD8 (upper panel) and CD4 (lower panel) T cells (see also ). The color codes for T cell subpopulations are as in (E).

Article Snippet: As part of the DENFREE initiative ( https://cordis.europa.eu/project/id/282378/results ) , we obtained the peripheral blood mononuclear cells (PBMCs) from two Thai male adult donors, both diagnosed with secondary DENV-4 infection with DF and DHF severities, aged 35 and 20 years old, and the viral loads of 1.76 x 10 6 and 1.77 x 10 7 , respectively ( ).

Techniques: Functional Assay, Infection, Expressing, Construct